The Comprehensive Analysis of the Typical Industrial Wastewaters via Physico-Chemical Method and Bioassay Using Luminescent Bacteria

Xiaoyan Y. Ma, Xiaochang C. Wang, HuuHao Ngo, Wenshan Guo, Na Wang, Maoni N. Wu, Xiaoting Wu



Five typical industrial wastewaters were evaluated with physico-chemical analysis, the acute toxicity and time-dependent toxicity assay using luminescent bacteria Vibrio qinghaiensissp.-Q67(Q67). The acute toxicity of the five industrial wastewaters in terms of EC50 are in an order of coking wastewater > electroplating wastewater > dyeing wastewater > gas-field wastewater > oil-field wastewater. The organic compounds in coking wastewater had the highest acute toxic effect on organisms, whereas the organic compounds in gas-field wastewater are less toxic. The acute toxicity of electroplating wastewater was contributed by heavy metals. Dying and oil-field wastewaters contained a certain concentration of organic compounds and heavy metals. Their dynamic toxicity characteristics were further investigated via time-concentration-effect surfaces obtained from the time-dependent toxicity assay and the efficiency of biological treatment of industrial wastewater was able to be predicted. For coking and electroplating wastewater treatment, the pretreatment or long hydraulic retention time (HRT) in biological treatment process was applicable. It was found that the substances withacute and chronic toxicityin dyeing and gas-field wastewaters were difficult to be degraded by bacteria. It should be aware that due to the weak acute toxicity and strong chronic toxicityofoil-field wastewater, the impactof this wastewater was easily underestimated.The results of time-dependent toxicity assay using bacteria provide another angle of view for industrial wastewater treatment.

Keywords: Industrial wastewater; luminescent bacteria; acute toxicity; time-dependent toxicity assay; biological treatment