Impact of 1-Hydroxybenzotriazole Dosing on Trace Organic Contaminant Degradation by Laccase

Luong N. Nguyen, Faisal I. Hai, Jinguo Kang, Saleh F. Magram, William E. Price, Long D. Nghiem




This study investigated the removal of five selected non-phenolic trace organic contaminants (TrOC) by extracellular enzyme extract from a white-rot fungus. Except diclofenac, no other investigated non-phenolic TrOC were degraded by the extracellular enzyme extract. Improvement of enzymatic degradation of all TrOC was achieved in the presence of a redox mediator, namely, 1-hydroxybenzotriazole (HBT). However, the enhancement of degradation was mediator concentration-specific. A significant improvement in degradation at higher dosage than 0.1 mM (HBT) was achieved.

Keywords: Trace organic contaminants; crude enzyme extract; laccase; mediator; 1-hydroxybenzotriazole (HBT)